Our Lab

Mechanisms of Behavior Lab introduces new Lab Director

Welcome note from David Sinkiewicz

It is a great privilege to have the opportunity to be the director of the Mechanisms of Behavior Lab here at Indiana University. Having spent the last 10 years developing my molecular biology and behavior skillsets I am eager to embrace this new challenge and opportunity to work with the great scientists associated with the Center for the Integrative Study of Animal Behavior.

During my training, as both a laboratory technician and as a graduate student at Georgia State University, I have had the opportunity to learn many techniques that have prepared me to work with the diverse set of research programs here at CISAB. My graduate studies have focused primarily on gene expression in brains of green treefrogs. This work was primarily focused on the development of a de novo brain transcriptome in this organism that is not traditionally used for genetic study. I also had the opportunity to collaborate with a fellow GSU lab in the production of the first de novo Syrian hamster brain transcriptome. While I have focused on these genetic techniques, I have also spent time extracting and analyzing steroid hormone levels in fish, birds, lizards, and frogs.

I am truly excited to be a part of such an impressive research center and to assist everyone in any way I can!

Mechanisms of Behavior Lab is now a CTSI Service Core

The mission of  Indiana Clinical and Translational Sciences Institute is to “create the highest-quality research dedicated to improving human health across Indiana and beyond.” CTSI Cores have demonstrated quality oversight, and users that span departments and schools, among other criteria. CISAB members whose research has clinical and translational applications are encouraged to explore the many programs CTSI has to offer, including the possibility of research funding opportunities. If you conduct labwork in the CISAB Mechanisms of Behavior Laboratory, consider applying for special CTSI Core Pilot Grants!

Services offered by the CISAB Lab

CISAB’s Mechanisms of Behavior Lab operates as a recharge center, providing the supplies and reagents required to perform a variety of common molecular and endocrine techniques, which are billed at cost upon completion. These include hormone extraction, enzyme immunoassay (EIA/ELISA), nucleic acid extraction, PCR, gel electrophoresis, and quantitative PCR preparation. Although we place a special focus on offering services valuable to IUB- and CISAB- affiliated researchers, non-affiliated researchers including those with research interests beyond animal behavior are also welcome to reach out and explore the possibilities of working in our lab. As a CTSI Designated Core, special funding opportunities may be available for researchers that conduct billable lab work.

View our billable techniques

CISAB Lab equipment

The following is a list of equipment currently available in the CISAB Lab. Please use the CISAB Lab Calendar to sign up for equipment ahead of time.

  • Water purification system (ultrapure)
  • Temperature controlled centrifuges (standard and micro)
  • Fume and laminar flow hoods
  • Electronic chemical balance
  • Dry heating block
  • Water baths (including shaking and sonication)
  • Standard and multi-tube vortexers
  • General-purpose incubator
  • Hot plate/stirrer
  • Refrigerators and -20°C and -80°C freezers
  • pH meter
  • Single-channel, multichannel and repeater pipettes
  • Cryostat
  • Microplate spectrophotometer with low-volume DNA/RNA/protein quantification
  • Rotor-stator homogenizer
  • Pellet pestle motor
  • Maxwell RSC nucleic acid purification system
  • Quantus Fluorometer
  • Thermocyclers (standard and gradient)
  • Vertical and Horizontal gel electrophoresis rigs
  • UV transilluminator with digital camera/printer
  • Western blot apparatus
  • Microplate absorbance plate readers
  • Microplate washers
  • Microplate shakers
  • Evap-o-rac for drying down samples under air or nitrogen
  • Vacuum manifold system